Search results for "Scratch assay"

showing 4 items of 4 documents

Formulation of liposomes loading lentisk oil to ameliorate topical delivery, attenuate oxidative stress damage and improve cell migration in scratch …

2021

[EN] Pistacia lentiscus L. is a sclerophyllous shrub capable of growing under harsh climatic conditions especially in the Mediterranean Basin. Different products can be obtained from this plant, such as essential oil, mastic gum or even fixed oil. The last is well known for its flavor which is mainly exploited in the food industry. Additionally, it has been traditionally used in the treatment of skin diseases, but, at the moment, any suitable formulation for skin delivery has been formulated and its biological effects was not deeply confirmed. Given that, in the present study, the lentisk oil has been formulated in liposomes at different concentrations (10, 20, 30 mg/ml) and their physicoch…

KeratinocytesSwineAdministration Topicalmedicine.disease_causelaw.inventionchemistry.chemical_compoundMiceSkin absorptionlawCell MovementScratch assayLecithinsMaterials TestingFood scienceHydrogen peroxideWound healingScratch assayFlavorLiposomeSkin permeation studiesbiologyVesiclePellGeneral MedicineOxidantsPistaciaKeratinocytes & fibroblastsPistacia lentiscusFarmacologiaKeratinocytes & fibroblastsDrug CompoundingWound healingRM1-950Cell LinemedicineOils VolatileAnimalsHumansAbsorció cutàniaParticle SizeEssential oilPharmacologyOlis essencialsPenetration (firestop)Hydrogen Peroxidebiology.organism_classificationConfocal microscopyOxidative StresschemistryEssences and essential oilsPistacia lentiscusLiposomesSkin permeation studies confocal microscopyTherapeutics. PharmacologySoybeansOxidative stress
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Extraction, Characterization and Incorporation of Hypericum scruglii Extract in Ad Hoc Formulated Phospholipid Vesicles Designed for the Treatment of…

2020

An extract of Hypericum scruglii, an endangered endemic plant of Sardinia (Italy), was prepared and characterized. It was loaded in special phospholipid vesicles, glycerosomes, which were modified by adding maltodextrin (glucidex) and a polymer (gelatin or hyaluronan). The corresponding liposomes were also prepared and used as reference. The vesicles disclosed suitable physicochemical features for skin delivery. Indeed, their mean diameter ranged from 120 to 160 nm, they were homogeneously dispersed (polydispersity index &le

food.ingredientlcsh:RS1-441Pharmaceutical Science02 engineering and technologyhypericaceaemedicine.disease_cause01 natural sciencesGelatinArticlelcsh:Pharmacy and materia medicagelatinhyaluronanchemistry.chemical_compoundglycerosomesfoodphospholipid vesiclesscratch assayZeta potentialmedicineoxidative stressHydrogen peroxideLiposome010405 organic chemistryVesiclekeratinocyte uptake021001 nanoscience & nanotechnologyMaltodextrinIn vitro0104 chemical scienceschemistryBiophysics0210 nano-technologyOxidative stress
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Multicomponent Antibiofilm Lipid Nanoparticles as Novel Platform to Ameliorate Resveratrol Properties: Preliminary Outcomes on Fibroblast Proliferati…

2023

The well-being of skin and mucous membranes is fundamental for the homeostasis of the body and thus it is imperative to treat any lesion quickly and correctly. In this view, polyphenols might assist and enhance a successful wound healing process by reducing the inflammatory cascade and the production of free radicals. However, they suffer from disadvantageous physico–chemical properties, leading to restricted clinical use. In this work, a complex mixture of PEGylated lipid, Glyceryl monoester, 18-β-Glycyrrhetinic Acid and Menthol was designed to entrap Resveratrol (RSV) as the active ingredient and further produce lipid nanoparticles (LNPs) by homogenization followed by high-freq…

Settore MED/07 - Microbiologia E Microbiologia Clinicaglycyrrhetinic acidantibiofilmOrganic Chemistrykinetic modelsmentholwound healingGeneral Medicinelipid nanoparticlesresveratrolCatalysisComputer Science ApplicationsInorganic ChemistrySettore CHIM/09 - Farmaceutico Tecnologico Applicativofibroblastsscratch assayresveratrol; glycyrrhetinic acid; menthol; lipid nanoparticles; drug release; kinetic models; wound healing; scratch assay; fibroblasts; antibiofilmPhysical and Theoretical ChemistrySettore BIO/06 - Anatomia Comparata E CitologiaMolecular BiologySpectroscopydrug release
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Entrapment of Citrus limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases

2020

This work aimed at developing a mouthwash based on liposomes loading Citrus limon var. pompia essential oil or citral to treat oropharyngeal diseases. Vesicles were prepared by dispersing phosphatidylcholine and pompia essential oil or citral at increasing amounts (12, 25 and 50 mg/mL) in water. Transparent vesicle dispersions were obtained by direct sonication avoiding the use of organic solvents. Cryogenic transmission electron microscopy (cryo-TEM) confirmed the formation of unilamellar, spherical and regularly shaped vesicles. Essential oil and citral loaded liposomes were small in size (~110 and ~100 nm, respectively) and negatively charged. Liposomes, especially those loading citral, …

0301 basic medicineLemonsSonicationkeratinocyte migrationlcsh:Medicinelcsh:RS1-441Pharmaceutical Sciencehydrogen peroxide02 engineering and technologyMalalties de la bocaCitral<i>Streptococcus mutans</i>law.inventionlcsh:Pharmacy and materia medica03 medical and health scienceschemistry.chemical_compoundlawPhosphatidylcholinescratch assayDrug DiscoveryLlimonesanticaries activityKeratinocyte migration<i>Citrus limon</i> essential oilHydrogen peroxideEssential oilLiposomeOlis essencialsChromatographyVesiclelcsh:RStreptococcusEstreptococs021001 nanoscience & nanotechnology030104 developmental biologyMouth diseaseschemistryEssences and essential oilsMolecular Medicine0210 nano-technology
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